Full length, sequence-verified cDNA clones
cDNA clones from transOMIC technologies provide the highest sequence quality and confidence when purchasing pre-made full length cDNA clones. Based on the Mammalian Gene Collection (MGC) developed by the National Institutes of Health (NIH) with rigorous sequence analysis resulting in less than 1 error in 50,000 bp (MGC Project Team 2009). All MGC premier
cDNA clones are backed by a 100% guarantee to be an exact match to the published sequence.
MGC premier cDNA clones have the following advantages:
• Ideal for native protein expression
• Full insert sequence easily available
• Best value on the market today
Full length, sequence-verified cDNA clones are available for human, mouse, rat, bovine, Xenopus and zebrafish genomes. These collections are available as individual cDNA clones, rearrays of cDNA clones for gene families and pathways as well as genome libraries.
MGC premier ORF clones
Over the course of the MGC project there was a transition to add functionality to the clones. One example is the production of Open Reading Frame (ORF) clones. These ORF clones lack the untranslated regions (UTRs) and have their native stop codon removed to allow the addition of peptide tags at the C- or N- terminus of the protein and are flanked by att sites for easy transfer to a destination expression vector via Gateway® reaction.
While these clones were created as part of the original cDNA clone project that produced the MGC premier
cDNA clones they are designated MGC premier
ORF clones in our gene search results and product literature to call out their unique value. Additional technical information can be found in the technical manual on the documents tab of this section. All MGC premier
ORF clones are clonally isolated and end-sequenced to confirm their identity before shipment. Turnaround time for shipment of these clones is 5 days.
Sequence verification service
MGC premier cDNA
clone identities can also be confirmed by end-sequencing prior to shipping. This risk-free option ensures the correct identity of the cDNA clone ordered.
transOMIC technologies will clonally isolate the cDNA clone/s ordered, end-sequence the requested clone/s and BLAST the output/s to eliminate any possible cross contamination that may be present from neighboring clones in the library. Successfully verified clones will be included in the shipment with their BLAST alignment. >95% identity over 100 bases of quality clipped sequence is considered a successful match to the desired clone.
Customers will be notified if a sequence fails and the item will be removed from their invoice. If an alternate clone for that gene is available it can be substituted for the incorrect clone.
To order this service use the Fetch my gene search to identify your cDNA clone of interest and select the item indicating it will be end sequenced before shipping.
Use the Fetch my Gene™
search tool to find and purchase
cDNA or ORF clones representing your gene of interest.
Gateway® is a registered trandemark of Life Technologies
Save time and money with pre-made MGC premier cDNA clones
Creating cDNA clones from biological material requires time, labor and money. While cDNA clone production is a well-established process it requires three major steps: synthesis, cloning and validation. These steps can delay a research project due to a number of variables including availability of biological material expressing the gene of interest, expression level of the gene, reverse transcription efficiency, amplification efficiency and ability to sequence verify the cDNA clone once it is created.
Pre-made MGC premier cDNA clone collections offer a convenient solution that decreases the time required to start an experiment and increases the chances of success. Rather than spending time on cloning, only a simple gene search using Fetch my Gene search tool is required.
Find and purchase your MGC premier cDNA clone today!